Seoul St. Mary's Hospital seoul, Seoul-t'ukpyolsi, Republic of Korea
Background/Case Studies: polycythemia vera (PV) is a chronic myeloproliferative neoplasm characterized by the JAK2V617F mutation. And Thrombotic and cardiovascular disease are the major causes of death in patient with PV. Confirmation of factors associated with thrombosis is needed to determine the most effective treatment for reducing the risk of thrombosis in patient with PV.
Study
Design/Methods: A total of 67 blood samples (37 for PV patients, 30 for controls) were investigated the characteristics of PV patients and controls. Also cell surface expression of Lu/BCAM (BV421 mouse anti-human BCAM, Becton-Dickinson) and Lw/ICAM (BV650 mouse anti-human ICAM-4, BD) were investigated using specific antibodies. LSRFortessaTM flow cytometer (BD) was used, as described.
Results/Findings: Among 37 PV patients, 27 were male and 10 were female. Age was 56 ± 15. 6. Hemoglobin (g/dL), WBC (109/L), platelet (109/L) counts were 16.0 ± 1.9, 9.4 ± 3.6, 371 ± 180, respectively. For controls, 17 were male and 13 were female. Age was 52 ± 13. 1. Hemoglobin (g/dL), WBC (109/L), platelet (109/L) counts were 15.0 ± 1.6, 5.4 ± 1.0, 240 ± 49, respectively. The mean fluorescence intensity (MFI) of Lu/BCAM and Lw/ICAM expression in PV patients was 3119 ± 1562, and 102 ± 39, respectively. MFI of Lu/BCAM and Lw/ICAM expression in controls were 2303 ± 1277, and 123 ± 61, respectively. Using the Mann-Whitney U-test, WBC, platelet counts and the expression of Lu/BCAM and Lw/ICAM between PV patients and controls showed P-value < 0.05. On Hemoglobin count, P-value was 0.056. Conclusions: Based on our results, we confirmed Lu/BCAM activation in PV patients as previously reported and decreased expression of Lw/ICAM. A decrease in expression of Lw/ICAM might be linked to the decrease action of erythroblastic islands in erythropoiesis. In Characteristics, PV patients showed higher WBC and platelet counts than controls.
Importance of research: Thrombotic and cardiovascular disease are the major causes of death in patient with PV and this research is related to it.
