(P-BB-44) Impact of Label Orientation on in vitro Parameters of Platelets During Storage

Background/Case Studies: In current standard practice, platelet units are stored face-label down to optimize gas exchange by maximizing surface area exposure. Noteworthy, DIN labels are applied to the opposite side of the platelet bag thereby reducing surface area available for gas exchange. This study addresses the impact of face-label position (up vs. down) on in vitro parameters of apheresis platelets during storage.

Study

Design/Methods: Double apheresis platelets were collected from six donors and stored in 100% plasma. Each product was split equally into two containers with identical (4 x 4 inch) face-label on one side and (3.5 x 3.5 inch) DIN labels on the opposite side. One unit was stored face-label up and the other was stored face-label down on a standard 22-24°C platelet agitator. Samples from each unit were tested on Day 1 and 7. Test parameters included platelet counts, blood gas analysis, morphology (Kunicki scoring), hypotonic shock response (HSR) and CD62P (P-selectin) expression.

Results/Findings: Two-way ANOVA analysis revealed no significant (p>0.05) differences between label positions. Platelet pH and glucose concentrations decreased as lactate increased with no change in platelet concentration during storage for both label positions. Platelet activation assessed by P-selectin expression increased over time in both face-label up and label down stored units. Additionally, there were no differences between label position for HSR or morphology scores which decreased during storage.

Conclusions: Storage bag label orientation did not impact in vitro platelet quality for 6 paired platelet units in 100% plasma. To standardize platelet management containers and minimize container handling, it is recommended that all products be stored face-label up during storage.

Importance of research: Platelet products are routinely stored with the label facing down to optimize surface area for gas exchange. Storing products with the label facing up has logistical advantages and may reduce container handling. The objective of this study was to assess the impact of the label position on in vitro platelet quality over 7 days of storage.