(P-BB-52) Interim Platelet Units Prepared from Automated WB Processing Meet In Vitro Acceptance Criteria and are Less Activated than Manually Prepared PRP Platelets

Background/Case Studies: Automated processing of whole blood (WB) into individual blood components may be more efficient than manual processing. This study compared the in vitro quality of interim platelet units (IPUs) derived from automated processing to manually prepared individual WB derived platelet (WBDP) units prior to leukoreduction and pooling.

Study

Design/Methods: WB was separated into components using the Reveos® Automated Blood Processing System (Terumo BCT, Inc., Lakewood, CO). Blood was processed 2-8 hours after collection into Reveos SELECT sets (Terumo BCT, Inc.) containing CPD anticoagulant. The individual WBDP units were manually prepared via the PRP method. The IPUs and WBDP units were evaluated using a standard panel of in vitro platelet quality assays. Reveos IPUs were sampled either on day 1 (N=28) or day 2 (N=37). All comparison WBDP units were sampled only on day 1 (N=67). The Reveos system and disposable set are not currently cleared for use in the US.

Results/Findings: The Reveos derived IPUs (inclusive of day 1 and day 2 units) meet primary endpoints for pH and platelet content in individual units, pH22°C ≥ 6.2. (95%/95%) and yield ≥ 5.5 × 10e10 platelets (75%/95%). The Reveos derived IPUs are within 20% of manual WBDP individual platelet units for all parameters shown except p-selectin (Table 1). The Reveos derived IPUs have lower mean p-selectin expression than manually processed WBDP units. The in vitro characteristics of IPUs sampled at day 1 vs day 2 are within 20% for all quality parameters except p-selectin and lactate.

Conclusions: Individual IPUs, prior to pooling and leukoreduction, derived from Reveos automated processing meet primary endpoints for pH and yield and have acceptable in vitro characteristics compared to manually prepared WBDP units.

Importance of research: Blood product quality depends on the collection procedure and post-collection processing. Use of automated systems for processing whole blood (WB) can improve productivity, efficiency, and consistency.